Regulatory

Part:BBa_K1895002:Design

Designed by: iGEM16_Newcastle   Group: iGEM16_Newcastle   (2016-09-06)


dnaK Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

[TODO]


Source

This sequence is taken from the Escherichia coli str. K-12 substr. MG1655, complete genome (sequence number [http://www.ebi.ac.uk/ena/data/view/U00096 U00096.3 in ENA]). We identified the sequence by reverse-translating the protein sequence given in UniProtKB ([http://www.uniprot.org/uniprot/P0A6Y8 P0A6Y8]) entry for dnaK in E. coli K-12.

References

Arsène, F., Tomoyasu, T. and Bukau, B., 2000. The heat shock response of Escherichia coli. International journal of food microbiology, 55(1), pp.3-9.

Ritossa, F., 1996. Discovery of the heat shock response. Cell stress & chaperones, 1(2), p.97.

Tilly, K., McKittrick, N., Zylicz, M. and Georgopoulos, C., 1983. The dnaK protein modulates the heat-shock response of Escherichia coli. Cell, 34(2), pp.641-646.